B-NDG Kit W41 mice
| Strain Name |
NOD.CB17-Prkdcscid Il2rgtm1Bcgen Kittm1(V831M)Bcgen/Bcgen
|
Common Name |
B-NDG Kit W41 mice |
| Background |
B-NDG |
Catalog number | 112914 |
| Aliases | Human: PBT, SCFR, C-Kit, CD117, MASTC | ||
|
NCBI Gene ID |
3815 | ||
Description
- C-Kit is expressed in various cells within the body, and it is significantly expressed in stem cells, progenitor cells, and other cells with self-renewal capabilities. In normal bone marrow, c-Kit is expressed by hematopoietic stem cells and plays a crucial role in self-renewal and differentiation into various blood cells. After binding with its ligand SCF, c-Kit undergoes autophosphorylation, thereby activating downstream signaling pathways and participating in cellular processes such as cell proliferation, differentiation, survival, adhesion, motility, and angiogenesis.
- Gene editing strategy: The 831 acid amino Val of Kit gene was mutated as Met in B-NDG Kit W41 mice.
- mRNA expression analysis: Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.
- Application: This product can accept human hematopoietic stem cell transplantation without irradiation, making it suitable for research in the fields of blood and immunity.
mRNA expression analysis
Strain specific analysis of Kit mRNA expression in B-NDG mice and B-NDG Kit W41 mice by RT-PCR. Spleen and lung RNA were isolated from B-NDG mice (+/+) and heterozygous B-NDG Kit W41 mice (Mut/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Kit primers. Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.
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